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Separation And Isolation Of Dna Fragment...

Separation And Isolation Of Dna Fragments

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The Process OF Recombinant DNA Technology || Isolation OF Genetic Material || Fragmentation OF DNA at a specific location by RE || Separation OF Isolation OF DNA Fragments

In the given process of separation and isolation of DNA fragments,some of the steps are missing,Complete the missing steps A: Restriction digestion of DNA fragments darr B: …………………… darr C: Staining with ethidium bromide darr D: Visualisation in U.V. light darr E: ............ darr F: Purification of DNA fragments.

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  • Following enzymes /techniques are used in the process of recombinant DNA technology A. Eco RI to cut the isolated genome B. DNA ligase C. Protease and ribonuclease for removal of proteins and RNA from DNA D. Production of recombination hosts E. lysozyme for isolation of the genetic material (DNA) F. gel electrophoresis for separation and isolation of DNA fragments Mark the correct sequence of their use.

    A
    C,E,B,F,A,D
    B
    E,C,A,B,F,D
    C
    E,C,A,F,B,D
    D
    A,E,C,B,D,F

  • Following enzyme /techniques are used in the proces of recombinant DNA technology A. EcorI to cut the isolated genome B. DNA ligase C. Protease and ribonuclease for removal of proteins and RNA and DNA C. Production of recombinant hosts E.Lysozyme for isolation of the genetic material (DNA) F. Gel electrophoresis for separation and isolation of DNA fragments Mark the correct sequence of their use

    A
    C,E,B,F,A,D
    B
    E,C,A,B,F,D
    C
    E,C,A,F,B,D
    D
    A,E,C,B,D,F

  • Which of the following is correct regarding the separation of DNA fragments during gel electrophoresis?

    A
    Smallest fragment will move to the farthest point towards cathode.
    B
    Smallest fragment will move to the farthest point towards anode.
    C
    Largest fragment will move to the farthest point towards anode.
    D
    Largest fragment will move to the farthest point towards cathode.

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    Processes OF rDNA Technology:- Introduction || Isolation OF DNA || Fragmentation OF DNA by RE || Separation and Isolation OF Desired DNA Fragment (Agarose Gel Electrophoresis)

    Processes OF rDNA Technology:- Introduction, Isolation OF DNA, Fragmentation OF DNA by RE, Separation and Isolation OF Desired DNA Fragment (Agarose Gel Electrophoresis)

    How isolation and separation of DNA fragments is done ?

    Gel electrophoresis is employed in . I. Separation of DNA fragmetns . II. Selection of DNA fragments . III. Vissualizing DNA IV. Check the progression of restriction enzyme digestion.

    Arrange the various steps of DNA fingerprinting technique in the correct order. (i) Separation of DNA fragments by electrophoresis. (ii) Digestion of DNA by restriction endonucleases. (iii) Hybridisation using labelled VNTR prode. (iv) Isolation of DNA . Detection of hybridised DNA fragments by auto-radiography. (vi) Transferring the separated DNA fragments to nitrocellulose membrane.

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