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Assertion : ELISA test is based on antig...

Assertion : ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins,glycoproteins, etc) on it
Reason : The pathogen antibody to be identified is immobilised on the surface of specially constructed ELISA plates and is then tested.

A

If both assertion and reason are true and reason is the correct explanation of assertion

B

If both assertion and reason are true but reason is not the correct explanation of assertion

C

If assertion is true but reason is false

D

If both assertion and reason are false

Text Solution

Verified by Experts

The correct Answer is:
D
ELISA (enzyme linked immuno-sorbant) is a technique based on the principle of antigen-antibody interactions. Pathogens have specific antigens on their surface like protein, glycoproteins, etc. Thus,their infection can be detected by using the antibodies specific to bind with those antigenes. In process antigens are immobilised to ELISA plates. Then antibody specific to the antigen is added and allowed to react with the adsorbed antigen. A complex procedure is followed.
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Knowledge Check

  • Assertion: ELISA is based on the principle of antigen antibody interaction Reason :Infection by pathogen can be detected by the presence of antigens or by detecting antibodies synthesized against the pathogen

    A
    If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
    B
    It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion
    C
    If Assertion is True but the Reason is False
    D
    If both Assertion & Reason are false

  • Widal test detects the presence of antibodies against which pathogen?

    A
    Vibrio cholerae
    B
    Paramyxovirus
    C
    Salmonella typhi
    D
    Influenza virus

  • Infection by a pathogen can be detected by the presence of antigens or by detecting the antibody synthesized against the pathogen. The test/s based on this principle is/are

    A
    PCR
    B
    ELISA
    C
    Both PCR and ELISA
    D
    Neither PCR nor ELISA

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