Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe.
I. Hybridisation using labelled VNTR prode.
II. Isolation of DNA.
III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
IV. Detection of hybridisation DNA fragments by autoradigraphy.
V. Separation of DNA fragments by electrophoresis.
VI. Digestion of DNA by restriction endonucleases.

Options

A. I, V, VI, II, III and IV
B. II, VI, V, II, I and IV
C. V, I, VI, III, IV and II
D. II, I, V, VI, IV, III

Answer

B

Explanation

The technique, as used earlier, involved Southern blot hydridisation using radiolabelled VNTR as a probe. It included
(i) Isolation of DNA,
(ii) Digestion of DNA by restriction endonucleases,
(iii) Separation of DNA fragments by electrophoresis.
(iv) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon,
(v) Hybridisation using labelled VNTR probe, and
(vi) Detection of hybridised DNA fragments by autoradiography.