Summarize the process by which the sequence of DNA bases in Human Genome Project was determined using the method developed by Frederick Sanger. Name a free living non-pathogenic nematode whose DNA has been completely sequenced.
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1. **Introduction to the Human Genome Project (HGP)**:
- The Human Genome Project was a large-scale research initiative aimed at sequencing the entire human genome, which consists of approximately 3 billion base pairs of DNA.
2. **Sequencing Method**:
- The sequencing of DNA bases was primarily accomplished using the Sanger sequencing method, developed by Frederick Sanger. This method is based on the principle of selective incorporation of chain-terminating dideoxynucleotides during DNA replication.
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