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In recombinant DNA technique, the term v...

In recombinant DNA technique, the term vector refers to :

A

plasmids that can transfer foreign DNA into a living cell

B

cosmids that can cut DNA at specific base sequence

C

plasmids that can join different DNA fragments

D

cosmids that can degrade harmful proteins

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### Step-by-Step Text Solution: 1. **Understanding Recombinant DNA Technology**: - Recombinant DNA technology involves combining DNA from different sources to create new genetic combinations. This is often used in biotechnology for various applications, such as producing insulin. 2. **Defining the Vector**: - In the context of recombinant DNA technology, a vector is a DNA molecule that is used to carry foreign genetic material into another cell. 3. **Types of Vectors**: - The most common vectors used are plasmids, which are small, circular pieces of DNA found in bacteria. Plasmids can replicate independently of the chromosomal DNA. 4. **Function of Vectors**: - Vectors serve as vehicles to transfer the gene of interest (such as the gene for insulin) into a host organism (like E. coli). Once inside the host, the vector allows the gene to be expressed and produce the desired protein. 5. **Key Features of Vectors**: - Vectors have specific features that make them suitable for gene transfer: - **Origin of Replication**: This allows the vector to replicate independently within the host cell. - **Selectable Markers**: These are genes that provide resistance to antibiotics, allowing for the identification of successfully transformed cells. - **Restriction Sites**: Vectors contain specific sequences where restriction enzymes can cut the DNA, allowing the insertion of the gene of interest. 6. **Conclusion**: - Therefore, in recombinant DNA techniques, the term "vector" specifically refers to plasmids or other DNA molecules that facilitate the transfer of genetic material into host cells, enabling the expression of foreign genes.
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Knowledge Check

  • In recombinant DNA technology, the term vector refers to

    A
    the enzyme that cuts DNA into restriction fragments
    B
    the sticky end of a DNA fragment
    C
    a plasmid used to transfer DNA into a living cell
    D
    a DNA fragment which carries only ori gene
  • In recombinant DNA technology, a plasmid vector is cleaved by

    A
    modified DNA ligase
    B
    a heated alkaline solution
    C
    the same enzyme that cleaves the donor DNA
    D
    the different enzyme than that cleaves the donor DNA.
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