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In recombinant DNA technique, the term v...

In recombinant DNA technique, the term vector refers to :

A

plasmids that can transfer foreign DNA into a living cell

B

cosmids that can cut DNA at specific base sequence

C

plasmids that can join different DNA fragments

D

cosmids that can degrade harmful proteins

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### Step-by-Step Text Solution: 1. **Understanding Recombinant DNA Technology**: - Recombinant DNA technology involves combining DNA from different sources to create new genetic combinations. This is often used in biotechnology for various applications, such as producing insulin. 2. **Defining the Vector**: - In the context of recombinant DNA technology, a vector is a DNA molecule that is used to carry foreign genetic material into another cell. 3. **Types of Vectors**: - The most common vectors used are plasmids, which are small, circular pieces of DNA found in bacteria. Plasmids can replicate independently of the chromosomal DNA. 4. **Function of Vectors**: - Vectors serve as vehicles to transfer the gene of interest (such as the gene for insulin) into a host organism (like E. coli). Once inside the host, the vector allows the gene to be expressed and produce the desired protein. 5. **Key Features of Vectors**: - Vectors have specific features that make them suitable for gene transfer: - **Origin of Replication**: This allows the vector to replicate independently within the host cell. - **Selectable Markers**: These are genes that provide resistance to antibiotics, allowing for the identification of successfully transformed cells. - **Restriction Sites**: Vectors contain specific sequences where restriction enzymes can cut the DNA, allowing the insertion of the gene of interest. 6. **Conclusion**: - Therefore, in recombinant DNA techniques, the term "vector" specifically refers to plasmids or other DNA molecules that facilitate the transfer of genetic material into host cells, enabling the expression of foreign genes.
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TRUEMEN BIOLOGY ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-MULTIPLE CHOICE QUESTIONS
  1. GAATTC is the recognization site for which of the following restrictio...

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  2. Plants in comparison to animals are more rapidly manipulated by geneti...

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  3. In recombinant DNA technique, the term vector refers to :

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  4. The mobile genetic element is

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  5. Which of the cell organelle lack membrane ?

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  6. Which one of the following palindromic base sequences in DNA can be ea...

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  7. Genetic engineering has been successfully used for producing:

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  8. In genetic engineering, a DNA segment (gene) of interest, is translate...

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  9. DNA or RNA segment tagged with a radioactive molecule is called

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  10. The vector for T-DNA is :

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  11. Which of the following is a plasmid ?

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  12. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  13. Which of the following is correctly matched?

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  14. PCR proceeds in three distinct steps governed by temperature they are ...

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  15. Restriction enzymes are used to cut

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  16. Which one of the following technique made it possible to genetically e...

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  17. Given below is a sample of a portion of DNA strand giving the base seq...

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  18. Agarose extracted from sea weeds finds use in

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  19. cDNA is

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  20. PCR and restriction Fragments length Polymorphism are the methods for

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