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Do eukaryotic cells have restriction end...

Do eukaryotic cells have restriction endonucleases? Justify your answer.

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Eukaryotic cells do not have restriction endonucleases, eukaryotic DNA is methylated by the enzyme methylase. This methylation prevents the action of endonucleases.
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Knowledge Check

  • Restriction endonucleases are

    A
    Used in genetic engineering for uniting two DNA molecules
    B
    Used for in vitro DNA synthesis
    C
    Present in mammalin cells for degeneration of DNA of dead cells
    D
    Synthesised by bacteria for their defence

  • Restriction endonuclease cuts

    A
    One strand of DNA at specific site
    B
    Both strands of DNA
    C
    Both strands of DNA at any site
    D
    Single strand of RNA

  • Restriction endonucleases used widely in RDT are obtained from

    A
    Plasmids
    B
    Bacterial cells
    C
    Bacteriophages
    D
    All prokaryotic cells

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