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Restriction endonuclease...

Restriction endonuclease

A

molecular build up at nucleotides

B

molecular degradation to DNA breakup

C

molecular knives for cutting DNA at specific sites

D

molecular cement to combine DNA sites.

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Read the given statements and select the correct option, Statement 1 : Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA Statement 2: Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme 1) α -glucosidase 2) restriction endonuclease 3) β -galactosidase 4) Taq polymerase

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (ii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector facilitates the gene cloning.

Match the scientists in column I with their related discoveries in column II and select the correct option from the given codes. {:(ColumnI, Column II),(A."Kary Mullis",(i)"Father of genetic engineering"),(B."Paul Berg",(ii)"Nobel prize for the discovery of restriction endonucleases"),(C."Stanley Cohen and Herbert Boyer",(iii)"Developed polymerase chain reaction"),(D."Arber smith and Nathan",(iv)"Isolated an antibiotic resistance gene from a plasmid of the bacterium Salmonella typhimurium"):}

How does a restriction nuclease function ? Explain.

Type-II Restriction enzymes cleave DNA fragments at which site

Restriction enzymes are used to cut single-stranded DNA.

Which of the following statements does not hold true for restriction enzyme? (a) It recongnises a palindromic nucleotide sequence (b) It is an endonuclease. (c) It is isolated from viruses (d) It produces the same kind of sticky ends in different DNA molecules

Differentiate between exonucleases and endonucleases.

Mention the difference in the mode of action of exonuclease and endonuclease.

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