After completing the transformation experiment involving the coding sequence of enzyme beta -galactosidase, the recombinant colonies should:

Options

A. give blue colour
B. not given blue colour
C. have active beta-galactosidase
D. both (2) and (3)

Answer

To answer the question regarding the expected outcome of recombinant colonies after completing the transformation experiment involving the coding sequence of the enzyme beta-galactosidase, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Role of Beta-Galactosidase**:
- Beta-galactosidase is an enzyme that breaks down lactose into glucose and galactose. It is often used as a reporter gene in molecular biology.

2. **Identify the Plasmid Used**:
- The transformation experiment is conducted using the plasmid PUC8, which contains the gene for beta-galactosidase.

3. **Insertion of DNA Sequence**:
- During the transformation, a foreign DNA sequence is inserted into the coding region of the beta-galactosidase gene. This process is known as cloning.

4. **Insertional Inactivation**:
- The insertion of the foreign DNA disrupts the normal coding sequence of beta-galactosidase, leading to the inactivation of the enzyme. This phenomenon is referred to as insertional inactivation.

5. **Expected Colony Color**:
- In the absence of the foreign DNA insertion, colonies that express functional beta-galactosidase will produce a blue color when grown on a medium containing a chromogenic substrate (like X-gal).
- However, when the beta-galactosidase gene is inactivated due to the insertion, the colonies will not produce the blue color.

6. **Conclusion**:
- Therefore, after completing the transformation experiment involving the coding sequence of beta-galactosidase, the recombinant colonies should not exhibit any blue color.

### Final Answer:
The recombinant colonies should not give any blue color.

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