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A foreign gene is inserted in pBR322 usi...

A foreign gene is inserted in pBR322 using the restriction
endonuclease EcoRl . Which of these is true about the E. coli having
this pBR322 ?

A

It will be resistant to both antibiotic ampicillin as well as antibiotic tetracycline

B

It will be resistant to antibiotic ampicillin but not resistant to
antibiotic tetracycline

C

It will be resistant to antibiotic tetracycline but not resistant to antibiotic ampicillin

D

It will be neither be resistance to antibiotic tetracycline nor to
antibiotic ampicillin

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question regarding the effects of inserting a foreign gene into the pBR322 plasmid using the restriction endonuclease EcoRI, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding pBR322**: - pBR322 is one of the first plasmids used for cloning. It contains genes that confer resistance to two antibiotics: ampicillin and tetracycline. 2. **Role of EcoRI**: - EcoRI is a restriction endonuclease that recognizes specific sequences in DNA and cuts the DNA at those sites. In the case of pBR322, the EcoRI site does not overlap with the genes responsible for antibiotic resistance. 3. **Insertion of Foreign Gene**: - When a foreign gene is inserted into pBR322 at the EcoRI site, it does not affect the existing antibiotic resistance genes for ampicillin and tetracycline. This is because the EcoRI site is separate from the resistance genes. 4. **Analyzing the Options**: - **Option A**: "It will be resistant to both antibiotics ampicillin as well as antibiotic tetracycline." - This option is correct because the insertion at the EcoRI site does not disrupt the resistance genes. - **Option B**: "It will be resistant to antibiotic ampicillin but not resistant to antibiotic tetracycline." - This option is incorrect. The plasmid remains resistant to both. - **Option C**: "It will be resistant to antibiotic tetracycline but not resistant to antibiotic ampicillin." - This option is also incorrect for the same reason as option B. - **Option D**: "It will be neither resistant to antibiotic tetracycline nor to antibiotic ampicillin." - This option is incorrect as well, since the plasmid retains its resistance. 5. **Conclusion**: - The correct answer is **Option A**: The E. coli containing the modified pBR322 will still be resistant to both ampicillin and tetracycline.
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