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"A probe is allowed to hybridize with it...

"A probe is allowed to hybridize with its complimentary DNA in a clone of cells and is later on detected by autoradiography".
Above step is the part of.

A

Recombinant DNA technology

B

Polymerase chain reaction

C

RNA interference

D

Gene therapy

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question, "A probe is allowed to hybridize with its complementary DNA in a clone of cells and is later on detected by autoradiography," we need to identify the process being described. Here’s a step-by-step breakdown: ### Step 1: Understand the Role of the Probe A probe is a short strand of nucleic acid that is labeled (often radioactively) and is used to detect the presence of complementary sequences of DNA. **Hint:** Think about what a probe does in molecular biology. ### Step 2: Identify the Hybridization Process Hybridization refers to the process where the probe binds to its complementary DNA sequence. This forms a hybrid molecule, which is crucial for the detection of specific DNA sequences. **Hint:** Consider what happens when two complementary strands of DNA come together. ### Step 3: Recognize the Formation of Recombinant DNA When the probe hybridizes with its complementary DNA, it forms a hybrid molecule. This hybridization is a key step in recombinant DNA technology, where DNA from different sources is combined. **Hint:** Remember that the term "recombinant" indicates the combination of DNA from different origins. ### Step 4: Detection Method - Autoradiography Autoradiography is a technique used to visualize the hybridized probe. It allows researchers to see where the probe has bound to the DNA by producing a detectable signal (like a band on a film) where the radio-labeled probe is located. **Hint:** Think about how scientists visualize specific molecules in a sample. ### Step 5: Conclusion Given the steps outlined above, the entire process described in the question is part of recombinant DNA technology. This technology involves the use of probes for hybridization and autoradiography for detection. **Final Answer:** The correct answer is **recombinant DNA technology**.
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Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:

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Knowledge Check

  • Which of the following statements are correct ? 1. PCR is now routinely used to detect HIV in suspected AIDS patients. 2. A single - stranded DNA or RNA , tagged with a radioactive molecule (probe) is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography. 3. ElISA is based on the principle of antigen - antibody interaction. 4. Today transgenic models exist for many human diseases such as cancer , cystic fibrosis , rheumatoid arthritis, and Alzheimer's

    A
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    B
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    C
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    D
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    A
    Chimeric DNA
    B
    Recoñbinant DNA
    C
    Probe
    D
    Vector DNA
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    A
    photographic film
    B
    autoradiography
    C
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    D
    replica plating
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