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In a polymerase chain reaction after the...

In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature ?
a) To permit specific annealing of the primers
b) To give a halt to the reaction mixture
c) To increase the activity of enzyme Taq polymerase
d) To obtain the multiple copies of the DNA

A

a) To permit specific annealing of the primers

B

b) To give a halt to the reaction mixture

C

c) To increase the activity of enzyme Taq polymerase

D

d) To obtain the multiple copies of the DNA

Text Solution

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The correct Answer is:
A
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Knowledge Check

  • The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?

    A
    B - denaturation at a temperature of about `98^@` C separating the two DNA strands
    B
    A - denaturation at a temperature of about `50^@`C
    C
    C-extension in the presence of heat stable DNA polymerease
    D
    A-annealing with two sets of primers

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