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In the isolation of DNA, removal of prot...

In the isolation of DNA, removal of protein and RNA is carried out by enzymes ______and_______respectively.
1) lysozyme, ribonuclease
2) protease, cellulase
3) protease, ribonuclease
4) ribonuclease, chitinase

A

1) lysozyme, ribonuclease

B

2) protease, cellulase

C

3) protease, ribonuclease

D

4) ribonuclease, chitinase

Text Solution

Verified by Experts

The correct Answer is:
C
In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. Since the DNA is enclosed by the membranes, we have to break the cell open to release DNA and other macromolecules like RNA, proteins, polysaccharides and lipids. it is obtained by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells) and chitinase (fungus). DNA is interwined with proteins like histones. RNA can be removed by treatment with ribonuclease while proteins can be removed by treatment with protease. Other molecules are removed by approprite treatment and purified DNA ultimately precipitates out after addition of chilled enthanol.
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Knowledge Check

  • 1. Enzymes (i) Proline 2. Amino acid (ii) A, B, C 3. Organic acid (iii) Protease, Lipase 4. Vitamins (iv) Acetic Acid

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