In which of the process the electric current of high voltage is applied to transfer foreign DNA

Options

A. Shot gun method
B. Micro injection method
C. Electroporation
D. biolistic method

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks about a process that uses high voltage electric current to transfer foreign DNA into cells.

2. **Analyzing the Options**:
- **Option 1: Shotgun Method**: This method is primarily used for sequencing DNA and involves breaking the genome into smaller fragments. It does not involve the transfer of foreign DNA using electric current.
- **Option 2: Micro Injection Method**: This method involves using a glass micro pipette to inject genetic material directly into a living cell. It does not involve the use of electric current.
- **Option 3: Electroporation**: This method applies an electric field to cells to increase their permeability, allowing foreign DNA to enter. This is the process that uses high voltage electric current for DNA transfer.
- **Option 4: Biolistic Method**: This method involves shooting DNA fragments into cells using a device known as a gene gun. It does not use electric current for the transfer.

3. **Identifying the Correct Answer**: Based on the analysis, the only method that involves the application of high voltage electric current to facilitate the transfer of foreign DNA is **Electroporation**.

4. **Conclusion**: The correct answer is **Electroporation**.

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