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A restriction enzyme called ECORI from ...

A restriction enzyme called ECORI from E. coli is expected to cleave DNA at the following sequence .

A

AAGTTC

B

GAATTC

C

AAGCTT

D

GTATATC

Text Solution

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The correct Answer is:
B
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The process of recombinant DNA technology has the following steps I. Amplification of the gene . II. Insertion of recombinant DNA in to the host cells . III. Cutting of DNA at specific location using restriction enzyme . IV . Isolation of genetic meterial (DNA) Pick out the correct sequence of step for recombinant DNA technology .

Restriction enzyme cleaves the _______ bond in DNA.

Knowledge Check

  • EcoRI cleaves DNA at

    A
    AGGGTT
    B
    GTATATC
    C
    GAATTC
    D
    TATAGC
  • EcoRI cleaves DNA at

    A
    AGGGTT
    B
    GTATATC
    C
    GAATTC
    D
    TATAGC
  • The process of recombinant DNA technology has the following steps I. Amplification of the gene. II. Insertion of recombinant DNA into the host cells. III. Cutting of DNA at specific location using restriction enzyme. IV.Isolation of genetic material orial (DNA)Pick out the correct sequence of step for recombinant DNA technology.

    A
    II, III, IV, I
    B
    IV, II, III, I
    C
    I, II, III, IV
    D
    IV, III, I, II
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    An intersting property of restriction enzymes is molecular cutting and past-ing. Restriction enzymes typically recognize a symmetrical sequence of DNA . Notice that the top strand is the same as the bottom strand, but reads backward . When the enzyme cuts the strand between G and A, it leaves over-hanging chains a. What is this symmetrical sequence of DNA known as ? b. What is the significance of these overhanging chains ? c. Name the restriction enzyme that cuts the strand between G and A.

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