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Rinku with a circular DNA contains seque...

Rinku with a circular DNA contains sequence
5' `larr` GGATTCC `to`3'
3' `larr` CCTTAAGG `to`5'
She wishes to add a new segment of DNA into it.
a. Identify the technology she planned.
b. Suggest the specific enzyme to make a cut in the DNA with above sequence.
c. Name the ctegory of enzyme your suggested.
d. How this enzyme identifies the sequence?
e. Draw the cut ends of the DNA with sequence.

Text Solution

Verified by Experts

a. Recombinant DNA technology
b. Restriction enzymes . e.g. EcoRI
c. Restriction endonucleases
d. Each restriction endonucleases recognises a specific palindromci nu-clecotide sequence.
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Knowledge Check

  • The enzyme used to join the DNA fragments is

    A
    topoisomerase
    B
    adenosine deaminase
    C
    DNA ligase
    D
    DNA polymerase
  • The enzyme used to cut the DNA molecule is

    A
    restriction endonucleases
    B
    2- galatosidase
    C
    DNA- ligases
    D
    DNA - polymerases
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