A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem
A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem
A
Red and green
B
Green and red
C
Orange and yellow
D
Purple and orange
Text Solution
AI Generated Solution
The correct Answer is:
To determine the color of the stained xylem and phloem in a transverse section of a stem stained first with safranin and then with fast green, we can follow these steps:
### Step-by-Step Solution:
1. **Understanding the Staining Process**:
- The transverse section of the stem is stained first with safranin, which is a red dye, and then with fast green, which is a green dye. This process is known as double staining.
2. **Staining with Safranin**:
- Safranin primarily stains lignified tissues and nuclei. In the context of the stem, the xylem, which contains lignin in its cell walls, will absorb the safranin and appear red.
3. **Staining with Fast Green**:
- Fast green stains cellulose-containing tissues. The phloem, which has cell walls made of cellulose, will absorb the fast green dye and appear green.
4. **Identifying the Colors of Xylem and Phloem**:
- After the double staining process, the xylem will retain the red color from safranin, while the phloem will take on the green color from fast green.
5. **Conclusion**:
- Therefore, in the prepared slide, the xylem will be red and the phloem will be green.
### Final Answer:
- The xylem will be stained red, and the phloem will be stained green.
To determine the color of the stained xylem and phloem in a transverse section of a stem stained first with safranin and then with fast green, we can follow these steps:
### Step-by-Step Solution:
1. **Understanding the Staining Process**:
- The transverse section of the stem is stained first with safranin, which is a red dye, and then with fast green, which is a green dye. This process is known as double staining.
2. **Staining with Safranin**:
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A transverse section of stem is stained first with safranin and then with fast green following the ususal schedule of double staining for the preparation of a permanent slide. What would be colour of the stained xylemand phloem?
A transverse section of stem is stained first with safranin and then with fast green following the ususal schedule of double staining for the preparation of a permanent slide. What would be colour of the stained xylemand phloem?
A
Red and green
B
green and red
C
orange and yellow
D
purple and orange.
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