A transverse section of stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem

Options

A. Red and green
B. Green and red
C. Orange and yellow
D. Purple and orange

Explanation

To determine the color of the stained xylem and phloem in a transverse section of a stem stained first with safranin and then with fast green, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Staining Process**:
- The transverse section of the stem is stained first with safranin, which is a red dye, and then with fast green, which is a green dye. This process is known as double staining.

2. **Staining with Safranin**:
- Safranin primarily stains lignified tissues and nuclei. In the context of the stem, the xylem, which contains lignin in its cell walls, will absorb the safranin and appear red.

3. **Staining with Fast Green**:
- Fast green stains cellulose-containing tissues. The phloem, which has cell walls made of cellulose, will absorb the fast green dye and appear green.

4. **Identifying the Colors of Xylem and Phloem**:
- After the double staining process, the xylem will retain the red color from safranin, while the phloem will take on the green color from fast green.

5. **Conclusion**:
- Therefore, in the prepared slide, the xylem will be red and the phloem will be green.

### Final Answer:
- The xylem will be stained red, and the phloem will be stained green.