Variation appearing in tissue culture are :

Options

A. Somaclonal variations
B. Clone variations
C. Somatic variations
D. Tissue culture variation

Answer

To answer the question "Variation appearing in tissue culture are:", we need to analyze the given options and identify which one accurately describes the variations that occur specifically in tissue culture.

### Step-by-Step Solution:

1. **Understanding the Options**:
- The options provided are:
- Somaclonal variations
- Clone variations
- Somatic variations
- Tissue culture variation

2. **Defining Somaclonal Variation**:
- Somaclonal variation refers to genetic variations that occur in plants as a result of tissue culture. These variations can manifest as changes in genetic or phenotypic traits. They are often observed in the number and structure of chromosomes.

3. **Defining Clone Variation**:
- Clone variation involves phenotypic and genetic variability within clonal populations. This variation can arise from the selection of host cells, generation of recombinant clonal cells, and their maintenance. However, it is not specifically tied to tissue culture.

4. **Defining Somatic Variation**:
- Somatic variation pertains to the diversity found in non-germinal tissues, which can occur due to mutations in somatic cells. This variation is not exclusive to tissue culture.

5. **Defining Tissue Culture Variation**:
- Tissue culture variation is a general term that does not specifically refer to the genetic changes that occur during tissue culture processes. It is too broad and does not pinpoint the specific type of variation we are looking for.

6. **Identifying the Correct Answer**:
- Among the options, somaclonal variation is the only term that specifically describes the genetic variations that arise as a direct result of plant tissue culture. Therefore, the correct answer is **Somaclonal variations**.

### Conclusion:
The variation appearing in tissue culture is **Somaclonal variations**.