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DNA staining by ethidium bromide is foll...

DNA staining by ethidium bromide is followed by the exposure to UV rays. Now DNA is seen as bands of

A

Blue colour

B

Orange colour

C

Red colour

D

Both B and C

Text Solution

Verified by Experts

The correct Answer is:
B

`**` the separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to ultraviolet radiation (we cannot see pure DNA fragments in the visible light and without staining)
`**` Bright organe coloured bands of DNA in a ethidium bromide stained gel exposed to UV light is observed
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Assertion: One can see bright orange coloured band of DNA in a ethidium bromide stained gel exposed to infra-red light Reason :One cannot see pure DNA fragments in the visible & UV light & without staining

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

After electrophoresis the separated DNA fragment can be vishualised ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

(A) : Ethidium bromide is a known mutagen. (R) : It can be used to stain DNA and visualise by exposure to UV radiation.

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

A2Z-BIOTECHNOLOGY: PRINCIPLES AND PROCESSES-Section A: Topicwise Questions (topic 2: Tools Of Tecombinant Dna Technology) Trestriction Enzymes ,
  1. Selectable markers are present in

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  2. In pBR 322 amp^(R) and "tet"^(R) gene are present. When we ligate a fo...

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  3. DNA staining by ethidium bromide is followed by the exposure to UV ray...

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  4. Which is responsible for controlling the copy number of the linked DNA...

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  5. Restriction' in restriction enzyme refers to

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  6. The role of DNA ligase in the construction of a recombinant DNA molec...

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  7. Recognise the figure and find out the correct matching

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  8. Genetic engineering has been made possible due to

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  9. Plasmids are used in genetic engineering because they are

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  10. Which statement is incorrect

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  11. Which of the following is a plasmid ?

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  12. Genes for antibiotic resistance located in Or Bacterial resistance...

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  13. When a recombinant dNA is inserted within the coding sequencing of an ...

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  14. In the E.coli cloning vector pBR 322, the number of selectable marker ...

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  15. Due to difficulty in inactivation of antibiotics, alternate selectable...

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  16. After the fragmentation by restriction endonuclease enzyme, the fragme...

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  17. In a chromosome there is a specific DNA sequence which is responsible ...

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  18. The separated bands of DNA are cut out from the agarose gel and extrac...

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  19. Restriction enzymes belong to a larger class of the enzyme called

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  20. In pBR 322, how many recognition sites are present ?

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