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Light of wavelength 5,000 Å is used in a...

Light of wavelength `5,000 Å` is used in a microscope of numerical aperture 0.10. Calculate the resovlving power of the microscope.

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To calculate the resolving power of the microscope, we can follow these steps: ### Step 1: Understand the formula for resolving power The resolving power \( R \) of a microscope is given by the formula: \[ R = \frac{2 \mu \sin \theta}{1.22 \lambda} \] where: - \( \mu \sin \theta \) is the numerical aperture (NA) of the microscope, - \( \lambda \) is the wavelength of light used. ### Step 2: Identify the given values From the problem, we have: - Wavelength \( \lambda = 5000 \) Å (angstroms), - Numerical aperture \( \mu \sin \theta = 0.10 \). ### Step 3: Convert the wavelength to meters Since the wavelength is given in angstroms, we need to convert it to meters: \[ \lambda = 5000 \, \text{Å} = 5000 \times 10^{-10} \, \text{m} = 5 \times 10^{-7} \, \text{m}. \] ### Step 4: Substitute the values into the formula Now, substituting the values into the resolving power formula: \[ R = \frac{2 \times 0.10}{1.22 \times 5 \times 10^{-7}}. \] ### Step 5: Calculate the denominator First, calculate the denominator: \[ 1.22 \times 5 \times 10^{-7} = 6.1 \times 10^{-7}. \] ### Step 6: Calculate the resolving power Now substitute this back into the formula for \( R \): \[ R = \frac{0.20}{6.1 \times 10^{-7}}. \] ### Step 7: Perform the division Calculating the division: \[ R = \frac{0.20}{6.1 \times 10^{-7}} = \frac{20 \times 10^{-1}}{6.1 \times 10^{-7}} = \frac{20}{6.1} \times 10^{6} \approx 3.2787 \times 10^{5} \, \text{m}^{-1}. \] ### Step 8: Round the final answer Rounding the answer gives: \[ R \approx 3.3 \times 10^{5} \, \text{m}^{-1}. \] ### Final Answer The resolving power of the microscope is approximately \( 3.3 \times 10^{5} \, \text{m}^{-1} \). ---
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Light of wavelength 5,000 Å is falling on a microscope objective lens with a cone angle of 60^@ . Calculate the resolving power of the microscope.

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Knowledge Check

  • Resolving power of light microscope is

    A
    `0.2 mum`
    B
    `0.1 mum`
    C
    `2 mum`
    D
    `100 mum`
  • Which colour should be used to increases the resolving power of a microscope

    A
    violet
    B
    red
    C
    yellow
    D
    green
  • A telescope uses light having wavelength 5000 Å and aperture of the objective is 10 cm, then the resolving limit and magnifying power of the telescope is respectively

    A
    `6.1xx10^(-6)` rad and 12
    B
    `5.0 xx10^(-6)` rad and 12
    C
    `6.1xx10^(-6)` rad and `8.3xx10^(-2)`
    D
    `5.0xx10^(-6)` rad and `8.3xx10^(-2)`
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