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One of the methods by which recombinant ...

One of the methods by which recombinant DNA can be introduced into host cell is by 'heat shock' treatment (electro-poration). Write down other three important methods by which we can introduce alien DNA into host cells .

Text Solution

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i. Micro-injection : Recombinant DNA is directly injected into the nucleus of animal cell.
ii. Biolistics or gene gun : Cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
iii. By disarmed pathogen vectors : These vectors are allowed to infact the cell, which transfers the recombinant DNA into the host .
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Knowledge Check

  • In genetic engineering, a DNS segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select correct option about which one or more of these can be used as vector/vectors. A) A bacterium B) Plasmid C) Plasmodium D) Bacteriophage

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