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One of the methods by which recombinant ...

One of the methods by which recombinant DNA can be introduced into host cell is by 'heat shock' treatment (electro-poration). Write down other three important methods by which we can introduce alien DNA into host cells .

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i. Micro-injection : Recombinant DNA is directly injected into the nucleus of animal cell.
ii. Biolistics or gene gun : Cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
iii. By disarmed pathogen vectors : These vectors are allowed to infact the cell, which transfers the recombinant DNA into the host .
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NEW JOYTHI PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -New Evaluation Type Questions
  1. Separation and isolation of DNA fragments is a major event in recombi-...

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  2. How are the DNA fragments separated through gel electrophoresis ex-tra...

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  3. Name two important cloning vectors .

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  4. What are the main features that are required to facilitate cloning in...

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  5. Explain the significance of origin of replication and selectable marke...

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  6. Name any three suitable 'selectable marker' for E.coli.

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  7. During genetic engineering experiments recombinants are distinguished ...

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  8. DNA is a hydrophilic molecule, so it cannot pass through cell membrane...

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  9. Number of recognition sites usually preferred for a vector is

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  10. You are provided with the mixture of transformed bacteria having recom...

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  11. Agrobacterium tumifaciens act as a natural genetic engineer , who tran...

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  12. Given below are the different steps in recombinant DNA technology. Arr...

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  13. DNA is usually intertwind with histone proteins and RNA. But in geneti...

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  14. Briefly explaing the 'cutting' and 'ligation' of DNA during recombinan...

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  15. One of the methods by which recombinant DNA can be introduced into hos...

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  16. Explain how the gene of interest is amplified invitro during biotechno...

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  17. What is the significance of thermostable DNA polymerase during PCR ? ...

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  18. Distinguish between recombinant DNA and recombinant protein.

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  19. Give the significance of using a 'bioreactor' in biotechnological expe...

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  20. Explain the importance of (a) ori, (b) amp^(R) and (c) rop in the E. c...

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