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Buffer Action

Buffer Action

A buffer is a mixture of substances that helps maintain a stable pH level in a solution, even when acids or bases are added to it.

1.0Define Buffer Action

Buffer action is known as the ability of a buffer solution to resist changes in pH when small amounts of acid or base are added. This is achieved through the neutralization reactions of the buffer components. When acid is added, it reacts with the base component of the buffer to form more of the weak acid, thus using up the added H+ ions and maintaining the pH. Conversely, when base is added, it reacts with the acid component of the buffer to form more of the weak base, neutralizing the OH ions and again stabilizing the pH. 

This dual action ensures that the system's pH remains relatively constant, essential for many biological and chemical processes.

Here are some important points to understand Buffer Action:

Neutralization of Acids: When a strong acid is added, its H+ ions are neutralized by the conjugate base in the buffer.

Neutralization of Bases: When a strong base is added, its OH ions are neutralized by the weak acid in the buffer.

pH Stability: These reactions help maintain a stable pH, preventing large swings.

Here is an image to demonstrate buffer action by showing how buffers stabilize pH. When acid is added:

Buffer Action

  • Water: pH drops from 7.0 to 3.2.
  • Buffer solution: pH slightly decreases from 7.0 to 6.8.

When base is added:

  • Water: pH rises from 7.0 to 11.5.
  • Buffer solution: pH slightly increases from 7.0 to 7.2.

Buffers maintain a stable pH despite the addition of acids or bases.

2.0Buffer Solution and Buffer Action

Buffer solutions and buffer action are directly related as they describe the mechanism and effect of stabilizing pH in a solution. A buffer solution, by its composition of a weak acid and its conjugate base (or weak base and its conjugate acid), inherently possesses the property of buffer action, which is its ability to resist changes in pH when acids or bases are added. This relationship is foundational in many biological and chemical systems, where maintaining a constant pH is crucial for the stability and functionality of processes and reactions.

3.0Acidic and Basic Buffer

Let's consider a buffer solution made from acetic acid (CH3COOH) and its salt, sodium acetate (CH3COONa). Sodium acetate dissociates in water to provide acetate ions (CH3COO).

Formation of the Buffer:

  • Sodium acetate dissociates in water: 

CH3COONa  →  CH3COO  +  Na+

  • Acetic acid partially ionizes in water: 

CH3COOH    ⇌   CH3COOー   +  H+

Case 1: Acidic Buffer

Adding Acid When you add a strong acid like HCl to this buffer, the H+ ions from the HCl would typically lower the pH by increasing the hydrogen ion concentration. However, in this buffer, the added H+ ions react with the acetate ions (CH3COO) to form more acetic acid (CH3COOH). This reaction consumes the H+ ions, minimizing any increase in the hydrogen ion concentration and thus stabilizing the pH.

The added H+ ions react with the acetate ions (CH3COO): 

H+ +  CH3COOー   →   CH3COOH

This reaction consumes the H+ ions, effectively minimizing the change in pH.

Case 2: Basic Buffer

Adding Base If you add a strong base like NaOH, the OH ions from the NaOH would typically raise the pH by reducing the hydrogen ion concentration. However, in the buffer, these OH ions react with acetic acid (CH3COOH) to produce water and more acetate ions (CH3COO). This reaction removes OHions from the solution, controlling any significant rise in pH.

The added OH ions react with acetic acid: 

OHー  +  CH3COOH  →  CH3COO  +  H2O

4.0Properties of Buffer Action

  1. Resistance to pH Change: Buffers effectively resist pH changes when a small amount of acid or base is added.
  2. Capacity: The buffer capacity known as the amount of acid or base the buffer can neutralize, depends on the concentrations of the buffer components.
  3. Optimal pH Range: Each buffer has an optimal pH range, typically within ±1 pH unit of the pKa of the acid in the buffer.

5.0Biological Buffers

Specialized buffer systems that operate in biological fluids to maintain pH levels vital for life processes. Examples include:

  1. Phosphate Buffer: Predominant in cells and plasma, helps in energy transfer and nucleic acid synthesis.
  2. Bicarbonate Buffer: Regulates blood pH, balancing carbon dioxide and water to form carbonic acid.
  3. Tris Buffer: Widely used in lab settings for DNA, RNA, and protein experiments due to its minimal interference with biological reactions.

6.0Applications of Buffer

Here are detailed examples of buffer applications across various fields:

  1. Biological Systems: 

In human blood, a bicarbonate buffer system helps maintain a pH of approximately 7.4. This system balances the effects of carbonic acid (H2CO3) and bicarbonate ion (HCO3) to stabilize pH, crucial for proper physiological functions.

  1. Medicine: 

Intravenous (IV) solutions often include phosphate buffers to maintain the necessary pH for drug stability and to ensure that the solutions are safe and effective when administered to patients.

  1. Industrial Applications: 

In dyeing processes, buffers are used to control the pH of dye baths to ensure that dyes bond properly to fabrics without damaging the material or altering the color.

  1. Chemical Research: 

In DNA extraction protocols, a Tris buffer is used to maintain a stable pH environment that protects the DNA from degradation while allowing enzymes like DNase to be inactivated.

  1. Agriculture: 

In hydroponics, buffers are added to nutrient solutions to prevent drastic pH changes, ensuring optimal nutrient availability and uptake by plants.

Frequently Asked Questions

Buffer action involves neutralizing added acids with the buffer's base component and neutralizing added bases with the buffer's acid component, thereby stabilizing pH.

Buffers maintain pH levels necessary for critical biological functions, such as enzyme activity and cellular processes.

Buffers have a limit to how much acid or base they can neutralize, known as their buffer capacity. This depends on the concentrations of the acid and base components in the buffer.

Common laboratory buffers include phosphate buffers, used in cell culture and molecular biology experiments, and Tris buffers, popular in protein and DNA work.

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