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The tripeptide (II) is:...


The tripeptide `(II)` is:

A

Gly `rarr` Leu `rarr` Val

B

Val `rarr` Leu `rarr` Gly

C

Leu `rarr` Gly `rarr` Val

D

Val `rarr` Gly `rarr` Leu

Text Solution

Verified by Experts

The correct Answer is:
B
(1) `DNP` method or Leucine aminopeptidase enzyme releasess `N-`terminal amino acid.
`underset((I))"Tetrapeptide"rarrunderset(("N-terminal"))("Alanine")+"Tripeptide"`
`underset(("N-terminal"))(Ala)rarr"Tripeptide"`
(2) Hydrazinolysis or carboxypeptidase enzyme releases `C-`terminal amino acid.
`underset((II))("Tripetide")rarr"Dipeptide"+underset(("C-terminal"))("Glycine")`
`underset(("N-terminal"))(Ala)rarr"Dipeptide"rarrunderset(("C-terminal"))(Gly)`
(3) Dipeptide `(III)` now contains only two amino acids, valine and Leucine. Edman method releases `N-`terminal amino acid.
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