COMPETENT HOST (FOR TRANSFORMATION WITH RECOMBINANT DNA)

INSERTION OF RECOMBINANT DNA INTO THE HOST CELL/ORGANISM

Amplification OF the Gene OF Interest using PCR|| Ligation OF the DNA fragment into a Vector|| Insertion OF Recombinant DNA into the Host cell/organism

What is recombinant DNA?

Tools Of Biotechnology-Competent Host |Processes Of Recombinant DNA Technology |OMR

Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction) || Ligation OF Desired DNA With Vector || Transformation OF Host cell With rDNA

Assertion Recombinant DNA can be chimeric DNA. Reason The chimeric DNA is that recombinant DNA, which is formed of two or more sources.

Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction), Ligation OF Desired DNA With Vector, transformation OF Host cell With rDNA

The term 'recombinant DNA' refers to

Assertion : Special methods are used for transformation i.e., incorporation of recombinant DNA into host. Reason : DNA is a hydrophilic molecule.

Which steps are involved in genetically modifying an organsim ? A. Identificaiton of desriable DNA B.Insertion of DNA into the host . C. Mainteance of introduced DNA in the host . D. Isolation of recombinant protein