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Oxidoreductases, tranferases , ligases ,...

Oxidoreductases, tranferases , ligases , papain.

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Papain - Enzymes present in papaya plant whereas others are microbial enzymes.
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What are DNA ligases?

Assertion : Genetic engineering requires both nuclease and ligases. Reason : Ligases produce the nick in the recombinant DNA molecule.

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1. Oxidoreductase (i) Digestive enzymes 2. Transferase (ii) Dehydrogenase 3. Hydro lases (iii) Decarboxylase 4. Lyase (iv) Transaminase

Find the odd one out of the following group. a. Thymine, Cytosine, Uracil, Adenine b. RNA primer, DNA polymerase, DNA ligase, DNA transcriptase c. Initiation, Elongation, Termination, Duplication d. AUG, UAG, UAA, UGA

Statements i. The four nucleotide bases are not necessarily present in DNA in exact equal proportions. ii. The total amount of purines are equal to the total amount of pyrimidines. iii. DNA ligase enzyme act to hydrolyse or break down a polynucleotide chain into its component nucleotides. iv. Nuclease enzymes are capable of restoring an intact DNA duplex. Of the above statements

Given table gives an account of differences between PCR and gene cloning. Which of the following points shows the incorrect difference ? {:("Parameter",PCR,"Gene cloning"),(1."Efficient","More","Less"),(2."Apparatus Requirement",DNA,"Restriction enzyme, ligase, vector, bacterial cell"),(3."Manipulation","in vitro","in vitro and in vivo"),(4."Cost","More","Less"),(5."Automation","Yes",No),(6."Error probability","Less","More"),(7."Time for a typical experiment","2-4days",4hours),(8."Application","More","Less"):} a) 1 and 3 b) 4,5 and 6 c) 4 and 7 d) 4,7 and 8

What is the role of DNA ligase in genetic engineering ?