Cloning

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Production of relplica of any cell or organ or entire organism is called cloning
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Which one of the following characteristics is generally not preferred for a cloning vector ? a) An origin of replication b) An antibiotic resistance marker c)Multiple restriction sites d) A high copy number

Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining. 1) Both statements 1 and 2 are correct 2) statement 1 is correct but statement 2 is incorrect 3) statement 1 is incorrect but statement 2 is correct 4) None of the above

Given table gives an account of differences between PCR and gene cloning. Which of the following points shows the incorrect difference ? {:("Parameter",PCR,"Gene cloning"),(1."Efficient","More","Less"),(2."Apparatus Requirement",DNA,"Restriction enzyme, ligase, vector, bacterial cell"),(3."Manipulation","in vitro","in vitro and in vivo"),(4."Cost","More","Less"),(5."Automation","Yes",No),(6."Error probability","Less","More"),(7."Time for a typical experiment","2-4days",4hours),(8."Application","More","Less"):} a) 1 and 3 b) 4,5 and 6 c) 4 and 7 d) 4,7 and 8

The gene 'rop' present in pBR322 cloning vector, codes for 1) the proteins involved in the translation 2) the proteins involved in the replication of the plasmid 3) the proteins involved in the synthesis of ampicillin only 4) the proteins involved in the synthesis of tetracycline only

Assertion : All expression vectors are cloning vectors and vise versa. Reason : Expression vectors have at least the regulatory sequences i.e., promotes, operators, ribosomal binding sites, etc having optimum function in the chosen control but not origin of replication.

Which of the following is the fist cloned organism?

Which one of the following processes results in the formation of clone of bacteria ?

Rashid isolated a natural plasmid from a bacterium and is planning to facilitate cloning . a. What are the minimum requirements for considering the isolated plas-mid as a vector ? How he identifies whether a foreign DNA is inserted or not after clon-ing ?

Read the following statements about asexual reproduction and select the correct ones. (i) It involves a single parent. (ii) It is slower than sexual reproduction. (iii) It produces progeny that are genetically identical with the parent but not with one another. (iv) The progeny of asexual reproduction can be termed as clones.

Consider the following statements : i. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro ii. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid. iii. Restriction enzymes belong to a class of enzymes called nucleases . Choose that correct option regarding above statements