Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

Write short notes on: Cloning sites

Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining.

Resbiction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.

What is the adventage of multiple cloning sites in vectors ?

Presence of more than one recognition sites within the vector will

Restriction enzyme cutting the same recognition site are called____.

Write the restriction site of EcoRI enzyme.