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If desired gene is inserted within the c...

If desired gene is inserted within the coding sequence of an enzyme, β-galactosidase in a vector then colonies of bacteria which do not produce any colour in the presence of a chromagenic substrates are :-

A

Recombinants

B

Transformants and non recombinants

C

Non transformants

D

Both (1) and (2)

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The correct Answer is:
A
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When a recombinant DNA is inserted within the coding sequencing of an enzymes, beta -galactosidase

What would happen if a recombinant DNA is inserted within the coding sequence of an enzyme, beta -galactosidase ?

True and false : In recombinant DNA, DNA inserted within the coding sequence of an enzyme beta galactosidase.

If recombinant DNA is inserted within the coding sequence of enzyme galactosidase, which of the following will occur in case of non -recombinants

After insertion inactivation of galactosidase gene which type of colonies do not produce any colour ?

If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme beta-galatosidase, he will separate out recombinants from non-recombinats by which of the following observations ?

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

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