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In the technique of recombinant insulin ...

In the technique of recombinant insulin production, the genes for `alpha`- and `beta`- polypeptides were inserted into the plasmid by the side of

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Which of the technique of recombinant insulin production uses insertion of genes for alpha and beta polypeptides in the plasmid by the sides of (A) Antibiotic resistance genes (B) lac-Z promoter gene (C) beta galactosidase gene (D) Ori

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

During gene cloning the enzyme used to join the insert DNA with the plasmid vector is :