A mixture contains three amino acids `A (pH = 3.2)`, `B (pH = 5.7)` and `C (pH = 9.7)`
When it is subjected to electrophoresis at pH 7.7, in which direction will each component of the mixture move?

Options

A. A to anode, B and C to cathode
B. A to anode, B no movement, C to cathode
C. A and B to anode C to cathode
D. A to cathode, B no movement, C to anode

Answer

C

Explanation

Since `pH` of `A` and `B` is less than `pH` (7.7), so they will exist in anionic form and will migrate towards anode.
Since `pI` of `C` is greater than the `pH` of medium, so it will exist in cationic form and migrate towards cathode.