Assertion : E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracyline.
Reason : Recoginition site for BamHI is present in `ter^(R)` region of pBR22.

Assertion: E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for Bam HI is present in "tet"^(R) region of pBR322.

Assertion : E.coli having pBR322 with DNA insert at BamH 1 site cannot grow in medium containing tetracycline. Reson: Recognition site for BamH 1 is present in tetR region of pBR322.

Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI

Assertion (A): Any fragment of DNA, when linked to the ori region, can be initiated to replicate. Reason (R) : Ori is a genetic sequence that acts as the initiation site for replication of DNA.

E. coli cloning vector pBR322 showing restriction sites ori and antibiotic resistance genes is given below. Select the correct option .

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector in used to transform Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vecotr will

The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:

The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides: