A plasmid has two antibiotic-resistance genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the one that

A plasmid has two antibiotic-resistance genes, one for tetracycline and other for ampicillin. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the ones that

A plasmid has two antibiotic-resistance genes, one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the ones that

The restriction enzyme used to cut bacterial plasmid and gene for human growth hormone is

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky-ends and these can be joined together (end to end) using

When a typical restriction enzyme cuts a DNA moleule, the cuts are uneven so that the DNA fragments have single-stranded ends . These ends are useful in recombinant DNA work because :

When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, so that the DNA fragments have single-stranded ends. These ends are useful in recombinant DNA work because

When DNA molecule is cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and these can be joined together (end to end) using

When DNA molecule is cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and these can be joined together (end to end) using

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases