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Study the given data and answer the ques...

Study the given data and answer the questions that follow.
A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample.
The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.
Two assays were carried out.
`{:("Lactose concentration (% w/v)",15,15,15,15,15,15),("Concentration of enzyme smaple (%v/v)",0,5,10,15,20,25),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,25,50,75,100,125):}`
`{:("Lactose concentration (% w/v)",0,5,10,15,25,30),("Concentration of enzyme smaple (%v/v)",5,5,5,5,5,5),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,15,25,35,40,40):}`
What are the variables in each of the two assays ?

A

`{:(" ""Assay"1," ""Assay"2),("Lactose concentration", "Conentration of enzyme sample"):}

B

`{:(" ""Assay"1," ""Assay"2),("Concentration of enzyme sample", "Lactose concentration"):}`

C

`{:(" ""Assay"1," ""Assay"2),("Lactose concentration", "Lactose concentration"):}`

D

`{:(" ""Assay"1," ""Assay"2),("Conentration of enzyme sample", "Conentration of enzyme sample"):}`

Text Solution

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The correct Answer is:
B
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Study the given data and answer the questions that follow. A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule. Two assays were carried out. {:("Lactose concentration (% w/v)",15,15,15,15,15,15),("Concentration of enzyme smaple (%v/v)",0,5,10,15,20,25),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,25,50,75,100,125):} {:("Lactose concentration (% w/v)",0,5,10,15,25,30),("Concentration of enzyme smaple (%v/v)",5,5,5,5,5,5),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,15,25,35,40,40):} Which of the following statements can be concluded from the two assays ?

Study the given data and answer the questions that follow. A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule. Two assays were carried out. {:("Lactose concentration (% w/v)",15,15,15,15,15,15),("Concentration of enzyme smaple (%v/v)",0,5,10,15,20,25),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,25,50,75,100,125):} {:("Lactose concentration (% w/v)",0,5,10,15,25,30),("Concentration of enzyme smaple (%v/v)",5,5,5,5,5,5),("Reaction rate "mu"mole glucose "sec^(-1)mL^(-1),0,15,25,35,40,40):} Which of the following assays would you expect to have the highest reaction rate ?

Lactose

Lactose is a disaccharide of

Lactose is a

Hydrolysis of disaccharide lactose gives

The ratio of the enzyme to substrate molecule can be as high as

Lactose is made of

 Enzyme lactase occurs inÂ

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