When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky-ends and these can be joined together (end to end) using

When DNA molecule is cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and these can be joined together (end to end) using

When DNA molecule is cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and these can be joined together (end to end) using

Restriction endonucleases are used to join the DNA fragments having sticky ends.

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

When a typical restriction enzyme cuts a DNA moleule, the cuts are uneven so that the DNA fragments have single-stranded ends . These ends are useful in recombinant DNA work because :