During DNA replication which of the following does not act as substrates?

Options

A. dATP
B. dCTP
C. dUTP
D. dGTP

Answer

### Step-by-Step Solution:

1. **Understanding DNA Replication**:
- DNA replication is the process by which a new DNA molecule is synthesized from an existing parent DNA molecule. This process occurs in the cytoplasm of prokaryotes and in the nucleus of eukaryotes.

2. **Identifying Substrates in DNA Replication**:
- During DNA replication, nucleotides are the building blocks or substrates that are incorporated into the growing DNA strand. The specific nucleotides used are deoxyribonucleotide triphosphates (dNTPs).

3. **List of Nucleotide Triphosphates**:
- The common substrates (dNTPs) used during DNA replication include:
- **DATP** (deoxyadenosine triphosphate)
- **DCTP** (deoxycytidine triphosphate)
- **DGTP** (deoxyguanosine triphosphate)
- **dTTP** (deoxythymidine triphosphate)

4. **Evaluating the Options**:
- The question provides four options, and we need to determine which one does not act as a substrate during DNA replication.
- **Option 1**: DATP - This is a substrate.
- **Option 2**: DCTP - This is also a substrate.
- **Option 3**: DUTP - This is not typically used in DNA synthesis; it is more associated with RNA synthesis.
- **Option 4**: DGTP - This is a substrate.

5. **Conclusion**:
- Based on the evaluation, the correct answer is **Option 3: DUTP**, as it does not act as a substrate during DNA replication.