For the strand separation and stabilisation during DNA replication which of the following set of enzymes and proteins are required?

Options

A. SSBP, gyrase , primase
B. Toposiomerase,helicase, ligase
C. Gyrase , ligase, primase
D. Topoisomerase, helicase, SSBP

Answer

To determine the set of enzymes and proteins required for strand separation and stabilization during DNA replication, we can analyze the options provided:

### Step-by-Step Solution:

1. **Understanding the Role of Enzymes and Proteins in DNA Replication**:
- DNA replication involves unwinding the double helix and stabilizing the separated strands to allow for the synthesis of new DNA.

2. **Evaluating the Options**:
- **Option 1: SSBP, Gyrase, Primase**:
- SSBP (Single-Stranded DNA Binding Protein) helps stabilize the unwound DNA strands.
- Gyrase (a type of Topoisomerase) helps relieve the tension caused by unwinding.
- Primase synthesizes RNA primers for DNA synthesis.
- This option is partially correct but does not include all necessary components for strand separation and stabilization.

- **Option 2: Topoisomerase, Helicase, Ligase**:
- Topoisomerase helps relieve supercoiling.
- Helicase unwinds the DNA double helix.
- Ligase connects DNA fragments but is not directly involved in strand separation or stabilization.
- This option is incorrect due to the inclusion of Ligase.

- **Option 3: Gyrase, Ligase, Primase**:
- Gyrase helps relieve supercoiling.
- Ligase connects DNA fragments.
- Primase synthesizes RNA primers.
- This option is incorrect as it does not include the necessary proteins for strand stabilization.

- **Option 4: Topoisomerase, Helicase, SSBP**:
- Topoisomerase helps relieve supercoiling.
- Helicase unwinds the DNA double helix.
- SSBP stabilizes the separated strands.
- This option includes all the necessary components for strand separation and stabilization.

3. **Conclusion**:
- The correct answer is **Option 4: Topoisomerase, Helicase, and SSBP**. These components work together to ensure proper strand separation and stabilization during DNA replication.