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A : Unknown DNA after hybridization wit...

A : Unknown DNA after hybridization with VNTR probe, the autoradiogram gives may bands of differing sizes in DNA profiling .
R: These bands represents DNA fingerpint of organism.

A

If both Assertion & Reason are true and the reason is the correct explanation of the assertion, then mark

B

If both Assertion & Reason are true and the reason is not the correct explanation of the assertion , then mark

C

If Assertion is true statement but Reason is false, then mark.

D

If both Assertion and Reason are false statements, then mark.

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Assertion :- In DNA fingerprinting, after hybridization with VNTR probe, the autoradiogram gives many bands of different sizes, Reason : It differes from individual to individual in a population except fraternal twins.

Study the given figure carefully and select the incorrect statements regarding this. (i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA (ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B (iii) The separated DNA fragments can be visualized after staining in the visible light (iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. this step is known as elution.

Hybridization is a concept of mixing or merging of orbitals of same atom with slight differences in energies to redistribute their energies and give new orbitals of equivalent energy called 'Hybrid Orbitals'. Hybridisation is a hypothetical concept and never actually exists. One should not be confused by a common misconception that hybridization is responsible for particular geometry. Geometry of a molecule is decided by energy factor not by hybridization. It is the orbital (which may be half filled, completely filled or empty) that undergoes hybridization and not the electron. The bond angles in hybridised orbitals are influenced by presence of lone pair, presence of multiple bonds, presence of one electron and electronegativity of atom. An increase in s-character of hybridised orbitals results in decrease in size of orbitals. This results in decrease in bond length and increase in energy. Among the following which have the same molecular geometry? (P) I_(3)^(-) (Q) XeF_(4) (R) BrF_(4)^(-) (S) XeO_(2)F_(2)

In DNA fingerprinting technique, radioactive DNA probe is obtained from ………………… of female banded krait snake.

How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.

AAKASH INSTITUTE-MOLECULAR BASIS OF INHERITANCE -ASSIGNMENT (SECTION -D) (Assertion -Reason Type Questions)
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  8. A : DNA fingerprinting are important to scientists in human genomic...

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  12. A : Kornberg enzyme is asscoitated with the removal of primers an...

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  13. A : Wobbling reduces the number of tRNAs required for polypeptides s...

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  14. A : Unknown DNA after hybridization with VNTR probe, the autoradiogr...

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  15. A: Polypeptide sequence are dictated by DNA and represented by mRNA. ...

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  16. A: Triplet genetic code can be confirmed by frame shift mutations. ...

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  17. A : Lac operon exerts negative control only. R: The operator is occ...

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  18. A : Single DNA depenedent RNA polymerase catalyases transcription of...

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  19. A : Sigma factor of RNA polymerase recognizes the start single regio...

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  20. A: HGP was completed in 2003 by sequencing all genes of all chrom...

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