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Gel electrophoresis is a...

Gel electrophoresis is a

A

technique of separation of charged molecules under the influence of magnetic field

B

technique of incorpotation of DNA molecules into the cell through transient pores made due to electrical impulses

C

technique of separation of DNA fragments through the pores of agarose gel under the influence of electric field

D

technique of separation and purification of gene products.

Text Solution

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The correct Answer is:
C

Electrophoresis is a technique of separation of molecules such as DNA, RNA or protein, under the influence of an electrial field, so that they migrate in the direction of electrode bearing the opposite charge, viz. positively charged molcules move towards cathode (-ve electrode) and negatively charged molecules travel towards anode (+ve electrode) through a medium/matrix. since DNA fragments are negatively charged molecules, they can be separated by allowing them to move towards the anode (+ve electrode) under an electric field through a matrix of agarose gel.
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Gel electrophoresis is employed in . I. Separation of DNA fragmetns . II. Selection of DNA fragments . III. Vissualizing DNA IV. Check the progression of restriction enzyme digestion.

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