Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes.
(iii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind ll was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition sites for a enzyme within the vector complicates the gene cloning.

Options

A. (i),(iii) and (v)
B. (ii) and (iv)
C. (iii), (iv) and (v)
D. (ii),(iii) and (iv)

Explanation

To determine which statements are correct, let's analyze each statement step by step.

### Step-by-Step Solution:

1. **Statement (i)**: "Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes."
- **Analysis**: This statement is incorrect. Different restriction enzymes recognize different sequences in DNA and produce different sticky ends. Sticky ends are specific to the recognition site of the enzyme used for cutting. Therefore, this statement is false.

2. **Statement (ii)**: "Exonucleases make cuts at specific positions within the DNA."
- **Analysis**: This statement is also incorrect. Exonucleases remove nucleotides from the ends of DNA molecules, not from specific internal positions. They work from the ends inward, so this statement is false.

3. **Statement (iii)**: "Hind II was the first restriction endonuclease to be isolated."
- **Analysis**: This statement is correct. Hind II, derived from the bacterium Haemophilus influenzae, was indeed the first restriction endonuclease to be isolated. Therefore, this statement is true.

4. **Statement (iv)**: "A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA."
- **Analysis**: This statement is correct. Bacteriophages can indeed infect bacterial cells and integrate their DNA into the host bacterial DNA, allowing them to replicate. Thus, this statement is true.

5. **Statement (v)**: "Presence of more than one recognition site for an enzyme within the vector complicates the gene cloning."
- **Analysis**: This statement is correct. If a vector has multiple recognition sites for a restriction enzyme, it can lead to the production of multiple fragments, making it difficult to isolate the desired gene of interest. Therefore, this statement is true.

### Summary of Correct Statements:
- Correct Statements: (iii), (iv), (v)
- Incorrect Statements: (i), (ii)

### Final Answer:
The correct statements are (iii), (iv), and (v).

---