Identify A,B,C, and D in the given figur...

Identify A,B,C, and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

`{:(A,B,C,D),("Hindl",EcoRl,amp^(R),"ori"):}`

`{:(A,B,C,D),("Hindl",BamHl,Kan^(R),amp^(R)):}`

`{:(A,B,C,D),(BamHl,Pstl,"ori",amp^(R)):}`

`{:(A,B,C,D),(EcoRl,BamHl,amp^(R),"ori"):}`

Text Solution

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`pBr322` was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning eperiments. In `pBR322` plasmid vector, p-denotes that it is a plasmid, `BR-` stands for Boliver and Rodriguez who constructed this plasmid, 322 - Is a number given to distinguish this plamid from others developed in the same laboratory. coil cloning vector `pBR 322` has restriction sites (Hindll, `EcoRl, BamHl, Sall, Pvull, Pstll, Clal)`, ori (origin of replication) and antibiotic resistance genes `(amp^(R)` and `tet^(R))`. rop codes for the proteins involved in the replication of the plasmid.

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Identify A,B,C,D in the given diagram of E.coli cloning vector pBR322.

`{:(A" "B" "C" "D),("Hindi 1"" ""EcoR1"" "amp^(R)" ""ori"):}`

`{:(A" "B" "C" "D),("Hindi 1"" ""BamH1"" "kan^(R)" "amp^(R)):}`

`{:(A" "B" "C" "D),("BamH1"" ""Pst 1"" "Ori" "amp^(R)):}`

`{:(A" "B" "C" "D),("EcoR 1"" ""BamH1"" "amp^(R)" ""ori"):}`

Identify the parts labelled as A, B, C, D and E in the given diagram of a human sperm and select the correct option.

A-Nucleus, B-Tail, C-Mitochondria,
D-Acrosome, E-Centriole

A-Acrosome, B-Nucleus,
C-Centriole, D-Mitochondria,
E-Plasma membrane

A-Nucleus, B-Mitochondria,
C-Plasma membrane,
D-Centriole, E-Neck

A-Acrosome, B-Centriole,
C-Mitochondria, D-Plasma membrane, E-Tail

In E.coli cloning vector PBR 322. ROP codes for -

The proteins involved in the replication of the plasmid

The proteins involved in the antibiotic resistance

The proteins involved in the synthesis of antibiotic

All of these