In pBR322, tetracycline resistance gene `(tet^(R))` has recognition site for which of the following restriction endonuclease ?

Options

A. Hindlll
B. BamHl
C. EcoRl
D. Pstl

Answer

B

Explanation

In plamid vector `pBR322`, two unique restriction sites Pstl and Pvul are located within the `amp^(R)` gene and BamHl, Sall, etc, are located within the `tet^(R)` gene. The presence of restriction sites within the marker genes `tet^(R)` and`amp^(R)` permits and easy selection for cells transformed with the recombinant `pBR322`. When restriction enzyme BamHl or Sal l is used, the DNA insert is placed within the gene `te^(R)` making it nonfunctional.