Home
Class 12
BIOLOGY
If a recombinant DNA bearing gene for re...

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as

A

selectable marker

B

recombinant protein

C

cloning site

D

chemical scalpels

Text Solution

Verified by Experts

The correct Answer is:
A

The cloning vector requires the presence of a selectable market, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants and selectively permitting the growth of the transformants. Normally, the geneus encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc. are considered useful selectable markers for E. coil. the normal E.coli cells do not carry resistance aganist any of these antibiotics.
Promotional Banner

Topper's Solved these Questions

  • BIODIVERSITY & CONSERVATION

    NCERT FINGERTIPS|Exercise Biodiversity & Conservation|140 Videos
  • BIOTECHNOLOGY AND ITS APPLICATION

    NCERT FINGERTIPS|Exercise Biotechnology And Its Application|137 Videos

Similar Questions

Explore conceptually related problems

If a recombinant DNA bearing gene for ampicillin resistance if transferred into E.Coli cells and the host cells are spread on agar plates containing ampicillin, then:

Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then

Assertion: The ampicillin resistance gene is called as selectable marker gene Reason :If host cells are spreaded on agar plates contraining ampicillin and only untransformed cells will survive

(a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it. (b) What does the ampicillin resistant gene act as in the above case?

The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc. are considered useful selectable markers in

Assertion : Selectrable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of transformants Reason: If a recombinant DNA bearing gene for resistance to ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin-resistant cells

NCERT FINGERTIPS-BIOTECHNOLOGY - PRINCIPLES AND PROCESSES-Biotechnology - Principles And Processes
  1. Given figures represents the steps involved in polymerase chain reacti...

    Text Solution

    |

  2. In a polymerase chain reaction after the denaturation step why the mix...

    Text Solution

    |

  3. If a recombinant DNA bearing gene for resistance to antibiotic ampicil...

    Text Solution

    |

  4. Which of the following is not used to transfer the recombinant DNA int...

    Text Solution

    |

  5. A device in which large volume of living cells are cultured in order t...

    Text Solution

    |

  6. Which of the following statements are correct with respect to a biorec...

    Text Solution

    |

  7. Identify the figures (A) and (B) and select the correct option.

    Text Solution

    |

  8. Stirred-tank bioreactors have advantages over shake flasks because the...

    Text Solution

    |

  9. After completion of the biosynthetic stage in the bioreactors, the pro...

    Text Solution

    |

  10. Study the following statements regarding recombinant DNA technology an...

    Text Solution

    |

  11. Read the following statements and select the incorrect ones. (i) Whe...

    Text Solution

    |

  12. Match the scientists in column I with their related discoveries in col...

    Text Solution

    |

  13. Select the correct option to fill up balnks. (i) is a natural polym...

    Text Solution

    |

  14. Four mutant strians of bacteria (1-4) all require substance S to grow ...

    Text Solution

    |

  15. The figure shows the restriction enzyme cutting sites (R1-R3) in wild ...

    Text Solution

    |

  16. Analyse the given diagram which steps involved in the procedure of sel...

    Text Solution

    |

  17. The nucleic acid extracted from animal liver is loaded and run on agar...

    Text Solution

    |

  18. The basic procedure involved in the synthesis of recombinanat DNA mole...

    Text Solution

    |

  19. Rising of dough is due is

    Text Solution

    |

  20. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

    Text Solution

    |