While isolating DNA from bacteria, which of the following enzymes is not used ?

Options

A. Lysozyme
B. Ribonuclease
C. Deoxyribonuclease
D. Protease

Explanation

To determine which enzyme is not used while isolating DNA from bacteria, we need to analyze the functions of the given enzymes in the context of DNA isolation.

### Step-by-Step Solution:

1. **Identify the Purpose of DNA Isolation**:
- The goal of isolating DNA from bacteria is to obtain pure DNA free from other cellular components such as proteins, RNA, and cell wall material.

2. **Examine Each Enzyme Option**:
- **Lysozyme**:
- Function: Lysozyme is an enzyme that breaks down the cell wall of bacteria. It is used to lyse bacterial cells to release their contents, including DNA.
- Conclusion: Lysozyme is used in DNA isolation.

- **Ribonuclease (RNase)**:
- Function: Ribonuclease is an enzyme that degrades RNA. During DNA isolation, it is important to remove RNA that may co-purify with DNA.
- Conclusion: Ribonuclease is used in DNA isolation.

- **Deoxyribonuclease (DNase)**:
- Function: Deoxyribonuclease is an enzyme that cleaves DNA by hydrolyzing phosphodiester bonds. This enzyme would degrade the DNA instead of isolating it.
- Conclusion: Deoxyribonuclease is not used in DNA isolation.

- **Protease**:
- Function: Protease is an enzyme that breaks down proteins. It is used to remove proteins that may contaminate the DNA sample.
- Conclusion: Protease is used in DNA isolation.

3. **Determine the Correct Answer**:
- Based on the analysis, the enzyme that is not used while isolating DNA from bacteria is **Deoxyribonuclease (DNase)**.

### Final Answer:
The enzyme that is not used while isolating DNA from bacteria is **Deoxyribonuclease (DNase)**.

---