Level of follicle stimulating hormone (FSH) during infancy and adulthood is the same but spermatogenesis is seen only during adulthood. mRNA levels coding for FSH receptors are also found to be same in testis of both age groups. Which of the following investigations will clarify this paradox a little more?

Options

A. Culture testicular cells and add LH to see testosterone production.
B. Culture testicular cells and add testosterone to see comparative rise in FSH mRNA from both age groups.
C. Culture testicular cells and FSH to see comparative rise in cAMP production by both age groups.
D. Add both LH and FSH to testicular cells and evaluate cAMP production.

Answer

A

Explanation

In males, FSH and LH are secreted by hypothalamus. FSH stimulates Sertoli cells of the testes to secrete androgen binding protein (ABP) that concentrates testosterone in seminiferous tubules LH stimulates interstitital cells (Leydig's cells) of the testes to secrete androgens testosterone. Androgens stimulate germinal epithelium to undergo spermatogenesis.