What would be the base sequence of RNA transcript obtained from the given DNA segment ?
5' - G C A T T C G G C T A G T A A C-3` Coding strand of DNA
3' - C G T A A G C C G A T C A T C A T T G-5' Non - coding strand of DNA

Options

A. 5'-G C A U U C G G C U A G U A A C-3'
B. 5'- C G U A A G C C G A U C A U U G -3'
C. 5'- G C A T T C G G C T A G T A A C -3'
D. 3'- C G T A A G C C G A T C A T T G-5'

Explanation

To find the base sequence of the RNA transcript obtained from the given DNA segment, we will follow these steps:

### Step 1: Identify the Coding Strand
The coding strand of DNA is given as:
5' - G C A T T C G G C T A G T A A C - 3'

### Step 2: Understand the Transcription Process
During transcription, the RNA polymerase reads the non-coding strand (template strand) of DNA and synthesizes RNA in the 5' to 3' direction. The RNA transcript will be complementary to the non-coding strand and will replace thymine (T) with uracil (U).

### Step 3: Write the Non-Coding Strand
The non-coding strand (template strand) is given as:
3' - C G T A A G C C G A T C A T C A T T G - 5'

### Step 4: Transcribe the Non-Coding Strand
To transcribe the non-coding strand into RNA, we will create a complementary RNA strand. The base pairing rules are as follows:
- C (cytosine) pairs with G (guanine)
- G (guanine) pairs with C (cytosine)
- T (thymine) pairs with A (adenine)
- A (adenine) pairs with U (uracil)

Using these rules, we transcribe the non-coding strand:
- C → G
- G → C
- T → A
- A → U
- A → U
- G → C
- C → G
- G → C
- A → U
- T → A
- C → G
- A → U
- T → A
- C → G
- A → U
- T → A
- T → A
- G → C

### Step 5: Construct the RNA Transcript
The RNA transcript, synthesized in the 5' to 3' direction, will be:
5' - G C A U U C G G C U A G U A A C - 3'

### Final Answer
The base sequence of the RNA transcript obtained from the given DNA segment is:
**5' - G C A U U C G G C U A G U A A C - 3'**

---