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Assertion : When the DNA sequences of tw...

Assertion : When the DNA sequences of two people are cut using the same restriction enzyme, the length and number of fragments obtained are different for both.
Reason : DNA sequence is arranged tandemly in many copy numbers which varies from chromosome to chromosome in an individual, showing high degree of polymorphism.

A

If both assertion and reason are true and reason is the correct explanation of assertion

B

If both assertion and reason are true but reason is not the correct explanation of assertion

C

If assertion is true but reason is false

D

if both assertion and reason are false.

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a
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When the genomes of two people are cut using the same restriction enzyme, the length and number of fragments obtained are different, this is called

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Assertion : Restriction enzyme cuts both vector DNA and foreign DNA at the same site Reason: Both plasmid and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

Assertion : Restriction enzymes cut the strand of DNA a little away from the centre of the palindromic sites Reason: Restriction enzymes cut the strads of DNA between the same two bases on the opposite strand.

Assertion DNA fingerprinting is very useful in forensic science. Reason It is the method, in which, individual pattern of DNA fragments is determined through the number and position of specific repeated sequences.

Assertion : Restriction endonuclease recognize short palindromic. Sequence and cut at specific sites. Reason : When a restriction endonuclease acts on Palindrome, it cleaves both the the strands of DNA molecules.

Read the passage given below and answer the question: EVIDENCE FOR THE FIBROUS NATURE OF DNA The basic chemical formula of DNA is now well established. As shown in Figure 1 it consists of a very long chain, the backbone of which is made up of alternate sugar and phosphate groups, joined together in regular 3' 5' phosphate di-ester linkages. To each sugar is attached a nitrogenous base, only four different kinds of which are commonly found in DNA. Two of these---adenine and guanine--- are purines, and the other two thymine and cytosine-are pyrimidines. A fifth base, 5-methyl cytosine, occurs in smaller amounts in certain organisms, and a sixth, 5-hydroxy-methyl-cytosine, is found instead of cytosine in the T even phages. It should be noted that the chain is unbranched, a consequence of the regular internucleotide linkage. On the other hand the sequence of the different nucleotides is, as far as can be ascertained, completely irregular. Thus, DNA has some features which are regular, and some which are irregular. A similar conception of the DNA molecule as a long thin fiber is obtained from physicochemical analysis involving sedimentation, diffusion, light scattering, and viscosity measurements. These techniques indicate that DNA is a very asymmetrical structure approximately 20 A wide and many thousands of angstroms long. Estimates of its molecular weight currently center between 5 xx 10^6 and 10^7 (approximately 3 xx10^4 nucleotides). Surprisingly each of these measurements tend to suggest that the DNA is relatively rigid, a puzzling finding in view of the large number of single bonds (5 per nucleotide) in the phosphate-sugar back bone. Recently these indirect inferences have been confirmed by electron microscopy. (source: Watson, J. D., & Crick, F.H. (1953, January). The structure of DNA. In Cold Spring Harbor symposia on quantitative biology (Vol. 18, pp. 123-131). Cold Spring Harbor Laboratory Press.) DNA molecule has _______ internucleotide linkage and ______ sequence of the different nucleotides

NCERT FINGERTIPS-MOLECULAR BASIS OF INHERITANCE-Molecular Basis Of Inheritance
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  2. If the base sequence of a codon in mRNA is 5'-AUG-3', the sequence of ...

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  3. The amino acid attaches to the tRNA at its

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  4. To initiate translation, the mRNA first binds to

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  5. In E. coli, the lac operon gets switched on when

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  6. Assertion : The sugar phosphatebackone of two chains in DNA double hel...

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  7. Assertion : In Griffith's experiment, a mixture of heat-killed virule...

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  8. Assertion : DNA isconsidered to be better gentic material than RNA for...

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  9. Assertion : The mechanism of DNA replication is semi-conservative in n...

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  10. Assertion : Synthesis of daughter or new srand accurs continuously al...

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  11. Assertion : Template or antisense strand , having 3' rarr 5' polarity...

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  12. Assertion : Polycistronic mRNA, found in prokaryotes, specify a number...

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  13. Assertion : Eukaryotic mRNA requires post transcription processing for...

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  14. Assertion : A change in nitrogen bass at the third position of a codo...

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  15. Assertion : tRNA recognises its corresponding codon in mRNA. Reason...

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  16. Assertion : Untranslated regions are sequences of RNA before initiat...

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  17. Assertion : The predominant site for control ofgene expression in pro...

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  18. Assertion : Lac operon is a repressible operon. Reason : The produc...

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  19. Assertion : Repetitive sequences make up very large portion of human g...

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  20. Assertion : When the DNA sequences of two people are cut using the sam...

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