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In a restriction digestion experiment, t...

In a restriction digestion experiment, the sticky ends of vector rejoined forming a circular vector without insert. Which enzyme can be used to eliminate this possibility?

A

DNA ligase

B

Alkaline phosphatase

C

DNA polymerase

D

RNA polymerase

Text Solution

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The correct Answer is:
B
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CENGAGE BIOLOGY-BIOTECHNOLOGY:PRINCIPLES AND PROCESSES-ARCHIVES (Choose the correct option)
  1. In a restriction digestion experiment, the sticky ends of vector rejoi...

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  2. The linking of antibiotic resistance gene with the plasmid vector beca...

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  3. Gel electrophoresis is used for

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  4. Polyethylene glycol method is used for

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  5. Restriction endonucleases are enzymes which

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  6. DNA or RNA segment tagged with a radioactive molecule is called :

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  7. Which one of the following palindromic base sequences in DNA can be ea...

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  8. Which one of the following is used as vector for cloning genes into hi...

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  9. Agarose extracted from sea weeds finds use in

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  10. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  11. PCR and restriction Fragements length Polymorphism are the methods for

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  12. Which one is a true statement regarding DNA polymerase used in PCR

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  13. For transformation, micro- particales coated with DNA to be bombarded ...

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  14. A single strand of nucleic acid tagged with a radioactive molecule is ...

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  15. which one of the following is a case of wrong matching?

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  16. Biolistics (gene-gun) is suitable for

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  17. In genetic engineering, the antibiotics are used

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  18. What is it that forms the basis of DNA Fingerprinting

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  19. Which one of the following represents a palindromic sequence in DNA ?

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  20. DNA fragments generated by restriction endonucleases in a chemical rea...

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  21. The colonies of recombinant bacteria appear white in contrast to blue ...

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