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The technique of transferring DNA fargme...

The technique of transferring DNA fargment separated on agarose gel to a synthetic membrane such as nitrocellulose is known as

A

Northern blotting

B

Southern blotting

C

Western blotting

D

Dot blotting

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The correct Answer is:
B
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Select the correct sequences of step in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as probe. I. Hybridisation using labelled VNTR prode. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridisation DNA fragments by autoradigraphy. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

Given below are the steps of DNA fingerprinting in random sequence:- (i) Isolation of DNA. (ii) Separation of DNA fragements by electrophoresis, (iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon. (v) digestion of DNA by restriction endonucleases, (vi). Detection of hybridised DNA fragments by auto radio-graphy. which of the following is the correct sequence:-

The technique used for separation of DNA fragment is:

The technique of extraction of DNA fragments from the gel is called:

Arrange these steps for DNA fingerprinting in the correct order: (I) Separated DNA fragments were transferred (blotting technique) to synthetic membranes, such as nitrocellulose or nylon. (II) Use of autoradiography for detection of hybridised DNA fragments. (III) DNA isolation: the DNA is isolated from the desired cells. (IV) Digestion of DNA by restriction endonucleases Order:

The DNA fragments separated on an agarose gel can visualised after staining with

The DNA fragments separated on an agarose gel can be visualised after staining with

Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

CENGAGE BIOLOGY-BIOTECHNOLOGY:PRINCIPLES AND PROCESSES-ARCHIVES (Choose the correct option)
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  3. Gel electrophoresis is used for

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  4. Polyethylene glycol method is used for

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  5. Restriction endonucleases are enzymes which

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  6. DNA or RNA segment tagged with a radioactive molecule is called :

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  8. Which one of the following is used as vector for cloning genes into hi...

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  10. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  11. PCR and restriction Fragements length Polymorphism are the methods for

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  12. Which one is a true statement regarding DNA polymerase used in PCR

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  13. For transformation, micro- particales coated with DNA to be bombarded ...

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  14. A single strand of nucleic acid tagged with a radioactive molecule is ...

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  15. which one of the following is a case of wrong matching?

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  16. Biolistics (gene-gun) is suitable for

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  17. In genetic engineering, the antibiotics are used

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  18. What is it that forms the basis of DNA Fingerprinting

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  19. Which one of the following represents a palindromic sequence in DNA ?

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  20. DNA fragments generated by restriction endonucleases in a chemical rea...

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  21. The colonies of recombinant bacteria appear white in contrast to blue ...

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