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A: Taq Polymerase is involved in PCR tec...

A: Taq Polymerase is involved in PCR technique.
R: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

A

If both Assertion and Reason are true and the reason is the correct explanation of the assertion, then mark (1).

B

If both Assertion and Reason are true but the reason is not the correct explanation of the assertion then mark (2).

C

If Assertion is true but Reason are false, then mark(3).

D

If both Assertion and Reason are false, then mark(4).

Text Solution

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The correct Answer is:
A
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Assertion: A thermostable DNA polymerase in used in PCR for repeated amplification Reason :It remain active during high temperature induced denaturation of ds DNA

A thermostable DNA polymerase, which remains active in high temperature induced denaturation of double-standed DNA is isolated from a bacterium

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the hight temperature induced denaturation of dsDNA. (ii) It required primers for carrying out the process of polymerisation. It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.

Which of the following statement are correct for the enzyme Taq polymerase? (i) It remains active during the high temperature . (ii) It requies primers for carrying out the process of polymerization. (iii) It induces denaturation of double-stranded DNA.

DNA polymerase or taq enzyme used in PCR is isolated from

Assertion (A) : Thermus aquaticus, is used in PCR technique. Reason (R): It is a heat-stable DNA polymerase.

In the following questions , a statement of assertion (A) is followed by a statement of reason (R). A:Thermostable enzyme Taq polymerase is used during annealing step of PCR.R: This heat tolerant enzyme helps primers to hydrogen bond to complementary DNA

Assertion (A): The enzyme involved in the continuous replication of DNA strand is DNA polymerase. Reason (R): The polarity of the template strand is 3 to 5' .

CENGAGE BIOLOGY-BIOTECHNOLOGY:PRINCIPLES AND PROCESSES-ARCHIVES (Choose the correct option)
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  2. The linking of antibiotic resistance gene with the plasmid vector beca...

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  3. Gel electrophoresis is used for

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  4. Polyethylene glycol method is used for

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  5. Restriction endonucleases are enzymes which

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  6. DNA or RNA segment tagged with a radioactive molecule is called :

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  7. Which one of the following palindromic base sequences in DNA can be ea...

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  8. Which one of the following is used as vector for cloning genes into hi...

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  9. Agarose extracted from sea weeds finds use in

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  10. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  11. PCR and restriction Fragements length Polymorphism are the methods for

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  12. Which one is a true statement regarding DNA polymerase used in PCR

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  13. For transformation, micro- particales coated with DNA to be bombarded ...

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  14. A single strand of nucleic acid tagged with a radioactive molecule is ...

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  15. which one of the following is a case of wrong matching?

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  16. Biolistics (gene-gun) is suitable for

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  17. In genetic engineering, the antibiotics are used

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  18. What is it that forms the basis of DNA Fingerprinting

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  19. Which one of the following represents a palindromic sequence in DNA ?

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  20. DNA fragments generated by restriction endonucleases in a chemical rea...

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  21. The colonies of recombinant bacteria appear white in contrast to blue ...

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