Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

The restriction site Pvu II is included in:

The restriction site Pvu II is included in:

" Number of restriction endonucleases of that can identify the recognition sequences of pBR322 is "

pBR322 has restriction sites for many restriction endonucleases. Which of these enzymes acting on pBR322 is not correctly matched with its source ? I. Pvul : Providencia vulgaris. II. Clal: caryophanon latum. III. Sall : Streptomyces albus IV. Pstl: Proteus stuarti

pBR322 has restriction sites for many restriction endonucleases. Which of these enzymes acting on pBR322 is not correctly matched with its source ? I. Pvul : Providencia vulgaris. II. Clal: caryophanon latum. III. Sall : Streptomyces albus IV. Pstl: Proteus stuarti

E. coli cloning vector pBR322 showing restriction sites ori and antibiotic resistance genes is given below. Select the correct option .

Assertion : E.coli having pBR322 with DNA insert at BamH 1 site cannot grow in medium containing tetracycline. Reson: Recognition site for BamH 1 is present in tetR region of pBR322.

Assertion: E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. Reason: Recognition site for Bam HI is present in "tet"^(R) region of pBR322.

At which of the following restriction sites foreign DNA should be ligated to inactivate tetracycline gene in a vector like pBR 322

Resistance genes present in pBR322 are