What procedure do you follow to understand the effect of plant growth hormones (in agar medium) in the terminal portion of the tip of stem (coleoptile) ?

Explanation

Aim : To show the growth of Avena coleoptile involves a chemical substance.
Apparatus : Oat seedlings (Avena sativa), coleoptile, a slice of agar, a sharp blade.
Procedure : Take some seedlings of coleoptile cut the tips by using a sharp blade. Place the tips on a slice of agar and leave them for about an hour. Cut the agar into small blocks and place a block on one side each stump of the decapitated plants. They were kept in the dark during the entire experiment. Observe the plants after one hour.
Observation : It is observed that seedling shows growth at the side on which agar block was placed.

Conclusion : It is proved that growing agents are secreted at the tip of stem and travel to bottom side, Which are called Auxins.